Pollito

Pollito is Eduardo's pet chicken and the secondary antagonist of the film. In Spanish, it literally means "Little Chicken".

Personality
When Lucy and Gru break into the Salsa & Salsa restaurant, Gru accidentally trips a wire which caused Pollito to come out. But Lucy and Gru don't think that the chicken is much of a threat. As Gru started insulting Pollito stating that the chicken is not so much of a guard dog, the chicken attacks and pecks the Gru's face. Lucy stops Pollito by shooting him with epoxy.

After the rough night Pollito recognizes Gru when he is at the restaurant picking up Margo. Polito immediately tries to attack, though Eduardo stops him. Instead of attacking, Pollito glares at Gru.

Polito is seen again when she spots Lucy at Eduardo's party, but instead of attacking Lucy he tries to get her purse. With help from Pollito, Eduardo finds out that Gru and Lucy work for the Anti-Villain League.

After defeating El Macho at the lair, Gru is trying to untie Lucy from El Macho's rocket, but Pollito presses the button that activates the rocket. This leads to Gru grumbling, "I really hate that chicken."

It is unknown what happens to Pollito after this master is arrested.